Contains chemical modifications to protect from degradation by cellular RNases.Target-specific RNA oligo, custom synthesized based on your sequence.
Option 1: Alt-R CRISPR-Cas9 crRNA:tracrRNA Alt-R CRISPR-Cas9 reagents and kits provide essential, optimized tools needed to use this pathway for genome editing research. After cleavage, DNA is then repairedīy non-homologous end-joining (NHEJ) or homology-directed recombination (HDR), resulting in a modified sequence. In an alternative approach, the crRNA and tracrRNA can be delivered as a single RNA oligonucleotide (Figure 1B). To activate the endonuclease and create a functional editing ribonucleoprotein complex (Figure 1A). Cas9 endonuclease requires a CRISPR RNA (crRNA) to specify the DNA target sequence, and the crRNA must be combined with the transactivating crRNA (tracrRNA) Target Capture Probe Design & Ordering ToolĬRISPR-Cas9 genome editing methods use a Cas9 endonuclease to generate double-stranded breaks in DNA.Library Concentration Conversion Calculator.Alt-R Predesigned Cas9 crRNA Selection Tool.SYBR Green dye assay and PrimeTime probe assays.PCR Allele Competitive Extension (PACE) genotyping.Drug target identification via CRISPR screening.
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